Back to All Enzymes

Vc1c

Host Organism

Viola canadensis

Type

PAL

Sequence

MATLVLLFLLAFSGFAAGGRDITGDGFLRLPSHGNGNSGDVDSKAGTKWAVLIAGSKGYQNYRHQADVCHAYQILRKGGVKDENIIVFMYDDIAYDIRNPYPGTIINSPDKKDVYKGVPKDYTGEDVNVQNFLAVILGNKTALTGGSGKVLDTRPNDHIFIYYTDHGAPGVLGMPTEPYLYANDLIDTLKKKHALGTYEGLVFYVEACESASIFEGLLPDGLNIYVSTAAKAGEGSWAAYCPSQEPPVPAEYGTCVGDLYSVTWMEDSDVYNLRTQTLHQQYELVKNKIAYASTVSQFGDFPISKDSLFEYMGTDPANEKRQYEDEEKSSSPHVGAVHQREADLHHFWDKYQKASEGSRNKVDARKQLVEVMLHRMHVDDSIESIAKLLFGSGAKASEMMNTIRPPGQPLVSDWDCLKTMVRTFETHCGSLSEYGMKYTRFLANICNSGIQKEKMGEASAQVCLNFP

External Links

PDB ID: 5ZBI
EC: 3.4.22.34
MEROPS: C13 legumain
UniProt ID: -
NCBI accession: -

Substrate Specificity

P1
P1'
P2'
N/D
X
L/I/F

Functions

  • Intermolecular backbone ligation
  • Backbone terminal cyclization, max. kcat/Km: 9.2 x 10^5 M-1s-1
  • Specificity stringency: Prime-low, Nonprime-high
  • pH optimal: pH 6.5

Expression Platform:

  • E. coli

Mutations:

  • Y168A/V238A

Other Properties:

-

References:

  1. [1] (Engineering) Chen, Y.; Zhang, D.; Zhang, X.; Wang, Z.; Liu, C. F.; Tam, J. P. Site-Specific Protein Modifications by an Engineered Asparaginyl Endopeptidase from Viola canadensis. Frontiers in Chemistry 2021, 9, 768854. DOI: 10.3389/fchem.2021.768854