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OaAEP3

Host Organism

Oldenlandia affinis (Roem. & Schult.) DC.

Type

PAL

Sequence

MVRYLAGAFQVVLLVVILSDIAISEERTDGYLKLPTEVSRFFRTPEQSSDGGDDSIGTRWAVLIAGSKGYDNYRHQADVCHAYQILKRGGLKDENIVVFMYDDIAYNESNPRPGVIINSPHGSDVYAGVPKDYTGDEVNAKNFLAAILGNKSAITGGSGKVVDSGPNDHIFIYYTDHGAPGVIGMPSKPYLYADELNDALRKKHASGTYKSMVFYLEACEAGSMFDGLLPDGLNIYALTASNTTEGSWCYYCPGQDAGPPPEYSVCLGDFFSIAWLEDSDVHNLRSETLNQQYHNVKNRISYASHATQYGDLKRGVEGLFLYLGSNPENDNYTFVDDNVVRPSSKAVNQRDADLVHFWEKFRKAPEGSSKKEEAQKQILEAMSHRVHIDSSINLIGKLLFGIEKGHKILTAVRSAGHPLVDDWACLRSLVRTFETHCGSLSQYGMKHTRTLANICNAGITEEQMAEAASQACVSIPSNPWSSHDGGFSA

External Links

PDB ID: -
EC: 3.4.22.34
MEROPS: C13 legumain
UniProt ID: -
NCBI accession: -

Substrate Specificity

P1
P1'
P2'
N/D
X
L/I

Functions

  • Intermolecular backbone terminal ligation
  • Backbone terminal cyclization
  • Specificity stringency: Prime-low, Nonprime-high

Expression Platform:

  • E. coli

Mutations:

-

Other Properties:

-

References:

  1. [1] (Sequence, activity) Harris, K. S.; Guarino, R. F.; Dissanayake, R. S.; Quimbar, P.; McCorkelle, O. C.; Poon, S.; Kaas, Q.; Durek, T.; Gilding, E. K.; Jackson, M. A.; et al. A Suite of Kinetically Superior AEP Ligases Can Cyclise an Intrinsically Disordered Protein. Sci. Rep. 2019, 9 (1), 10820. DOI: 10.1038/s41598-019-47273-7